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Unlike oligo(dT) primers, these primers usually are not template unique and they are ideal for DNA synthesis utilizing Klenow fragments with DNA templates or for cDNA synthesis working with reverse transcriptase with mRNA templates.But I was also contemplating a talent realized that might maintain me capturing really should there be a lack (which T

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bps on the three' conclusion. Enable This necessitates at least one primer (for a specified primer pair) to get the required amount of mismatches to unintended targets. The larger the mismatches (In particular These toward three' conclusion) are involving primers as well as the unintended targets, the more certain the primer pair is to your templat

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